Hsa_circ_001680 affects the proliferation and migration of CRC and mediates its chemoresistance by regulating BMI1 through miR-340

摘要

Circ_001680 is overexpressed in colorectal cancer. Circ_001680 is generated from the B2M gene, located on chromosome 15. Upper: Sequencing results showing the back-splice junction sequences of circ_001680. Lower: circ_001680 sequences that were PCR-amplified from cDNA and gDNA with divergent primers, respectively. Relative expression levels of circ_001680 in 42 paired human colorectal cancer tissues. The Mann-Whitney test was used to detect the relative expression level of circ_001680 in 42 normal and noncancerous tissues(***  e Correlation between circ_001680 expression and the stage of clinical T classification in 42 cases of CRC and noncancerous tissues (**  p f Graphical illustration of the statistical circ_001680 distribution in CRC patients. (**  p g qRT-PCR analysis of circ_001680 expression in 11 CRC cell lines