Elucidation of the time-dependent degradation process in insolublehyaluronic acid formulations with a controlled degradation rate

摘要

Degradation rate of hyaluronic acid to prolong its stability in vivo would bebeneficial. We investigated a potential solution for prolonging the stability ofhyaluronic acid within the body. We focused on decreasing the swelling ratio toslow the degradation rate of hyaluronic acid by insolubilizing sodiumhyaluronate without using potentially harmful substances such as crosslinkers ormodifiers. Hyaluronic acid formulations were created with three differentswelling ratios and time-dependent morphological changes in hyaluronic acidformulations and were scored based on each swelling ratio. In vivo degradationwas modeled in simulated body fluid and the extent of decay of test membraneswere monitored over time. Results showed that, by adjusting the swelling ratio,the degradation rate of hyaluronic acid formulation could be controlled. Ourresearch could lead to improvements in many products, not only preventivematerials for postoperative adhesions, but also pharmaceutical products such asosteoarthritis treatments and cosmetic medicines.