Transcriptional responses of LSm14A after infection of blue eggshell layerswith Newcastle disease viruses

摘要

LSm14A is a key innate immunity component of processing body (P-body) that mediatesinterferon-β (IFN-β) signaling by viral RNA. This is the first study to report chickenLSm14A (cLSm14A) cloning from layer, and chickens. The cLSm14A gene, encoding 461amino acids, is highly homologous in the three types of chickens. The cLSm14A wasextensively expressed in several tissues. The transcriptional level of cLSm14A wassignificantly increased in various stages of Newcastle disease virus (NDV) infection. InHEK293 cells, full length cLSm14A from layer was localizedin the cytoplasm as dots. The results of this study indicated that cLSm14A is an importantsensor that mediates innate immunity in chicken against NDV infections.