Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometryidentification of Mycobacterium bovis in Bovinae

摘要

In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF)mass spectrometry was used to identify from cattleand buffalo tissue isolates from the North and South regions of Brazil, grown in solidmedium and previously identified by Polymerase Chain Reaction (PCR) based on Region ofDifference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extractionprotocol and the mass spectra reference database were optimized for the identification of80 clinical isolates of mycobacteria. As a result of this optimization, it was possible toidentify and differentiate from other members of the complex with 100% specificity, 90.91%sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein providessuccessful identification of within bovine/bubaline clinicalsamples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.