Does HOTAIR expression level in the peripheral blood have veritably predictive/prognostic impact on breast cancer patients?

摘要

Schematic representation of the quantitative amplification plot and melt curve for molecule in cDNA samples, HOTAIR molecule in cDNA samples and molecule in genomic DNA samples. Image also schematically shows qualitative fragment analyses of the indicated PCR products. The samples 1, 2 and 3 are respectively representative images of normal individual, breast cancer patients before and after radiotherapy (10 Gy). A specific fragment was determined for (146 bp), while no fragment was generated from HOTAIR amplification in cDNA samples (potential cDNA fragment length: 197–201 bp depending on the different HOTAIR transcript variants; potential genomic fragment length: 389 bp). A specific fragment was also detected for (389 bp) in genomic DNA of all representative samples. NTC; Non-template control