PSIV-31 Late-Breaking: Effects of dietary multigrain carbohydrase in lactation diets on immune responses and gut microbiota of lactating sows and their litters.

摘要

The study evaluated the effects of dietary multigrain carbohydrase in lactation diets on immune response and gut microbiota of lactating sows and their litters. A total of 12 sows (218.37 ± 5.5 kg BW; 2.0 parity) were randomly assigned to 2 dietary treatments: a diet based on corn-soybean meal (CON) and CON supplemented with 0.01% multigrain carbohydrase. Sows were fed the dietary treatments for 28 days (lactation) after farrowing. Blood samples were collected from sows on d 0, 3, and 7 after farrowing and randomly selected 2 nursing piglets in each sow on d 3, 7, and 14 after birth. Measurements were serum tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), C-reactive protein (CRP), cortisol and immunoglobulin G, M, and A by the enzyme-linked immunosorbent assay. Three sows per dietary treatment and two nursery pigs were randomly selected to collect feces on d 7 and 28 after farrowing to verify their microbiota changes by pyrosequencing analysis. Data were analyzed using the PROC GLM procedure of SAS. There were no differences in immune response of lactating sows between CON and MCS. However, TNF-α of nursery pigs from sows fed MCS was increased on d 3 of lactation (244.14 vs. 282.11 pg/ml; P < 0.05) compared with those from sows fed CON. Nursing pigs from MCS-sows had lower CRP on d 7 of lactation (128.65 vs. 92.63 ng/ml; P < 0.05) than those from CON-sows. No differences were detected on TGF-β, cortisol and immunoglobulin G, M, and A of nursery pigs between dietary treatments. During lactation period, MCS increased (P < 0.05) genus Lactobacillus in gut microbiota of sows and their litters compared with CON. In conclusion, addition of dietary multigrain carbohydrase in the lactation diet influenced immune responses and gut microbiota of lactating sows and their litters.