The PCA Biosensor in . . Representation of the biosensor for detection of protocatechuic acid (PCA) is shown. Engineered chimeric promoter-operator (P ) inserted upstream from the eGFP reporter gene. PcaV binds to the promoter-operator sequence(s) repressing the expression of the reporter gene. Upon PCA binding to PcaV derepression occurs generating a fluorescent signal. PCA biosensor dose response curves. eGFP gene expression data in the absence (empty shapes) and presence of the PcaV repressor (filled shapes), for the P (red triangles) and P (green squares) versions of the PCA biosensor in . . Titration curves for active screened compounds using the P version of the PCA biosensor in and their respective effector. The fluorescent gene expression normalized to cell density (RFU/OD ) is shown relative to increasing concentrations of the effector. Each value represents the mean and standard deviation of 3 biological replicates
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