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Electrochemical immunosensor based on carboxylated single‐walled carbon nanotube‐chitosan functional layer for the detection of cephalexin

机译:基于羧化单壁碳纳米管-壳聚糖功能层的电化学免疫传感器检测头孢氨苄

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摘要

In this study, a sensitive and selective electrochemical immunosensor for cephalexin (CEX) determination on a glassy carbon electrode (GCE) surface was modified by a carboxylated single‐walled carbon nanotubes/chitosan (SWNTs‐COOH/CS) composite. The SWNTs‐COOH/CS composite was used to enhance sensor performance and to enlarge the electrochemical response of CEX. The cephalosporin‐ovalbumin coupling (CEX‐OVA) was synthesized using the reactive ester method. The free CEX in solution could be effectively measured based on the competitive immunoreaction between CEX‐antibody and CEX. Under optimal conditions, the electrochemical immunosensor offered an excellent response for CEX. The linear range was 1–800 ng/ml, with a detection limit of 45.7 ng/ml (  = 3). This method was applied to determine CEX in six different samples and obtained the recovery range from 80.15% to 94.04%. These results indicated that the fabricated electrochemical immunosensor and sensing method are suitable for quantification of CEX in real samples. These have great potential for wider applications in environmental and agri‐food products industries.
机译:在这项研究中,通过羧化单壁碳纳米管/壳聚糖(SWNTs-COOH / CS)复合材料修饰了一种用于测定玻璃碳电极(GCE)表面上的头孢氨苄(CEX)的灵敏选择性电化学免疫传感器。 SWNTs-COOH / CS复合材料用于增强传感器性能并扩大CEX的电化学响应。头孢菌素-卵清蛋白偶联(CEX-OVA)使用反应性酯法合成。根据CEX抗体和CEX之间的竞争性免疫反应,可以有效地测量溶液中的游离CEX。在最佳条件下,电化学免疫传感器为CEX提供了出色的响应。线性范围为1–800 ng / ml,检测极限为45.7 ng / ml(= 3)。该方法用于测定六个不同样品中的CEX,回收率从80.15%到94.04%。这些结果表明,所制备的电化学免疫传感器和传感方法适用于定量真实样品中的CEX。这些在环境和农业食品工业中具有广阔的应用潜力。

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