Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia Belgium

摘要

is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the genus or even the family, such as A23, , (Y), and D1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the isolates, including isolates retrieved from the same farm. might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential.