Crotonylation of key metabolic enzymes regulates carbon catabolite repression in Streptomyces roseosporus

摘要

Immunoblot of lysate. Twenty μg of total protein were loaded, and the crotonylated proteins were detected with anti-Kcr monoclonal antibody (1:2000) while Coomassie blue staining was used for the loading control. Immunoblot for crotonylation with anti-Kcr monoclonal antibody (1:2000) in wild-type strain cultured in TSB with additional concentration of sodium crotonate (pH 7.4) or sodium acetate (pH 7.4) as indicated for 24 h. Twenty μg of total protein were loaded, and Coomassie blue staining was used for the loading control. LC-MS analysis of cellular crotonyl-CoA and acetyl-CoA levels from cells cultured as in . The data represent mean peak area ±SD of three independent experiments. value was calculated with Student’s test (ns means  > 0.05).