Lysine Acetylome in Streptomyces roseosporus Reveals the Roles of Lysine Acetylation in Regulating Biosynthesis of Secondary Metabolites

摘要

1,143 Kac sites in 677 acetylated substrates were identified in S. roseosporus proteome. Its acetylome profile shows great similarity with gram + bacteria, E coli, B. subtilis, and S. enterica, suggesting S. roseosporus acetylated proteins are highly conserved. Functional classification of the 667 lysine acetylated proteins indentified in this study demonstrates that they are associated with a broad range of functions in S. roseosporus, such as various metabolic pathways, transport, transcriptional and, translational levels of gene expression. For example, 249 proteins are known to be associated with multiple metabolic pathways, including glycolysis, TCA cycle, carbohydrate metabolism and amino acid biosynthesis were lysine acetylated in S. roseosporus. This study is the first to show the proteins involved in secondary metabolite biosynthesis are lysine acetylated. Further investigation of the biological relevance of the acetylation will be needed to reveal the molecular mechanism of acetylation regulation in S. roseosporus. Our data provide a critical basis to elucidate the role of acetylation in Streptomyces metabolites biosynthesis.