Prevalence of 3.7 and 4.2 deletions in Sudanese patients with red cells hypochromia and microcytosis

摘要

Multiplex PCR products on agarose gel electrophoresis. Lane 1 and 13 were the DNA ladder (1 Kb). Lane 2 and 3 were the 3.7 and 4.2 heterozygous control samples respectively. Lane 12 was the negative control. Lane 4, 5, 7, 9, 10 and 11 were examples of negative sample result. Lane 6 and 8 were example of heterozygous of 3.7 deletion mutation. LIS1 primer was used as internal control for the amplification of the LIS1 as a house keeping gene and the product band size was of 2.5 kb. The 3.7 and 4.2 deletions and the normal α product bands size were 2.1, 1.6 and 1.8 kb respectively