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Isolation and characterization of cephalothin-susceptible Campylobacter coli from slaughter cattle.

机译:屠宰牛头孢菌素敏感性弯曲杆菌的分离与鉴定。

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摘要

In a recent meat survey, 10 of 13 (77%) Campylobacter coli isolates were susceptible to cephalothin. These organisms were isolated from nine slaughter cattle from eight meat packing establishments. All 10 isolates grew at 43 degrees C but not at 25 degrees C, were catalase and oxidase positive, and were susceptible to nalidixic acid (30 micrograms) and cephalothin (30 micrograms). The cultures were subsequently identified as C. coli serogroup 20, biotype I (Lior scheme). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that protein and lipopolysaccharide profiles of whole cell preparations of the 10 cephalothin-susceptible strains and the reference strain for C. coli serogroup 20 were very similar. The plasmid profiles of these 11 strains were identical.
机译:在最近的肉类调查中,在13株弯曲杆菌中,有10株(占77%)易受头孢菌素的影响。这些生物是从八个肉类包装企业的九头屠牛中分离出来的。所有10个分离株均在43摄氏度而不是25摄氏度下生长,过氧化氢酶和氧化酶呈阳性,并且对萘啶酸(30微克)和头孢菌素(30微克)敏感。随后将培养物鉴定为C. coli血清群20,生物型I(Lior方案)。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示,10例对头孢菌素敏感的菌株和C. coli血清群20的参考菌株的全细胞制剂的蛋白质和脂多糖谱非常相似。这11个菌株的质粒图谱是相同的。

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