The role of the β5-α11 loop in the active-site dynamics of acylated penicillin-binding protein A from Mycobacterium tuberculosis

摘要

Penicillin-binding protein A (PBPA) is a class B penicillin-binding protein that is important for cell division in M. tuberculosis. We have determined a second crystal structure of PBPA in apo form and compared it with an earlier structure of the apo enzyme. Significant structural differences in the active site region are apparent, including increased ordering of a β-hairpin loop and a shift of the SxN active site motif such that it now occupies a position that appears catalytically competent. Using two assays, including one that uses the intrinsic fluorescence of a tryptophan residue, we have also measured second-order acylation rate constants for the antibiotics, imipenem, penicillin G and ceftriaxone. Of these, imipenem, which has demonstrable antitubercular activity, shows the highest acylation efficiency. Crystal structures of PBPA in complex with the same antibiotics were also determined and all show conformational differences in the β5-α11 loop near the active site, but these differ for each β-lactam and also for each of the two molecules in the crystallographic asymmetric unit. Overall, these data reveal the β5-α11 loop of PBPA as a flexible region that appears important for acylation and provide further evidence that PBPs in apo form can occupy different conformational states.