Building Fluorescent DNA Nanodevices on Target Living Cell Surfaces

摘要

We report 1) the anchoring of preformed fluorescent DNA nanodevices (NDs) and 2) the in situ self-assembly of fluorescent DNA NDs on target living cell surfaces. Three types of aptamer-tethered DNA NDs were built and anchored on target cell surfaces by specific target-aptamer association. The in situ nanodevice self-assembly was further demonstrated on the surfaces of target living cells in cell mixtures. These DNA NDs exhibited fluorescence emission and underwent fluorescence energy transfer on living cell surfaces.