AGING OF ADHESIVE INTERFACES TREATED WITH BENZALKONIUM CHLORIDE AND BENZALKONIUM METHACRYLATE

摘要

Inhibition of endogenous dentin matrix metalloproteinases (MMPs) within incompletely infiltrated hybrid layers can contribute to the preservation of resin-dentin bonds. This study evaluated the bond stability of interfaces treated with benzalkonium chloride (BAC) and benzalkonium methacrylate (MBAC), and its inhibitory properties in dentin MMP activity. Single-component adhesive ALL-BOND UNIVERSAL, modified with BAC or MBAC in concentrations of 0, 0.5, 1.0 and 2.0% was used for microtensile bond strength (μTBS) evaluation after 24 h, 6 months and 1 yr. Human dentin beams were treated with 37% phosphoric acid, dipped either in 0.5% BAC, 1.0% BAC or water (control) for 60 s and then incubated in SensoLyte generic MMP substrate to determine MMP activity. A significant decrease in μTBS after 6 months and 1 yr was observed for the control group only. No significant differences among groups were shown at 24 h. After 6 months and 1 yr, the control group demonstrated significantly lower μTBS than all treatment groups. Both 0.5% and 1.0%, BAC applied for 60 s inhibited total MMP activity by 31% and 54%, respectively. Both BAC and MBAC contributed to the preservation of resin-dentin bonds likely due to its inhibitory properties of endogenous dentin proteinases.