Inhibition of endogenous dentin matrix metalloproteinases (MMPs) by benzalkonium chloride (BAC) decreases collagen solubilization and may help improve resin-dentin bond stability.This study evaluated the resin-dentin bond stability of experimental adhesive blends containing BAC and the stability of dentin matrices by assessing the mass loss and collagen solubilization from dentin beams pretreated with BAC.Twenty-five healthy molars were used for the bond strength evaluation of a two-step etch-and-rinse adhesive (Adper Single Bond Plus, SB) modified with BAC or not. The following groups were tested: 1) SB with no inhibitor (control); 2) topical 2.0% chlorhexidine + SB; 3) 1.0% BAC etchant + SB; 4) 0.5% BAC-SB; and 5) 1.0% BAC-SB. Microtensile bond strength (μTBS) and failure mode distribution under standard error of the mean were evaluated after 24 hours and six months of storage in artificial saliva (AS). A two-way analysis of variance and Tukey test with a significance level of p<0.05 was used for data analysis. In addition, 30 completely demineralized dentin beams from human molars were either dipped in deionized water (DW, control) or dipped in 0.5% and 1.0% BAC for 60 seconds, and then incubated in AS. Collagen solubilization was assessed by evaluating the dry mass loss and quantifying the amount of hydroxyproline (HYP) released from hydrolyzed specimens after four weeks of incubation.The control group demonstrated lower μTBS than some of the experimental groups containing BAC at 24 hours and six months (p<0.05). When BAC was incorporated into the adhesive blend in concentrations of 0.5% and 1.0%, no reduction in dentin bond strength was observed after six months (p<0.05). Less mass loss and HYP release was seen for dentin matrices pretreated with BAC relative to the control pretreated with DW (p<0.05).This in vitro study demonstrates that BAC contributes to the preservation of resin-dentin bonds by reducing collagen degradation.
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机译：苯扎氯铵（BAC）对内源性牙本质基质金属蛋白酶（MMP）的抑制作用可降低胶原蛋白的增溶作用，并可能有助于改善树脂-牙本质键的稳定性。本研究通过BAC评估了含BAC的实验性胶粘剂混合物的树脂-牙本质键稳定性和牙本质基质的稳定性。评估BAC预处理牙本质束的质量损失和胶原增溶作用。使用25颗健康磨牙评估是否经过BAC改性的两步蚀刻和冲洗粘合剂（Adper Single Bond Plus，SB）的粘合强度。测试了以下组：1）没有抑制剂的SB（对照）; 2）局部用2.0％洗必泰+ SB; 3）1.0％BAC蚀刻剂+ SB; 4）0.5％BAC-SB; 5）1.0％BAC-SB。在人工唾液（AS）中储存24小时和6个月后，评估平均标准误差下的微拉伸粘合强度（μTBS）和破坏模式分布。数据分析使用p <0.05的显着性水平的方差和Tukey检验的双向分析进行。此外，将30颗来自人体磨牙的完全脱矿质的牙本质束浸入去离子水（DW，对照）中，或浸入0.5％和1.0％BAC中60秒，然后在AS中孵育。孵育四周后，通过评估干重损失并量化水解标本中释放的羟脯氨酸（HYP）量来评估胶原蛋白的增溶作用。对照组在24小时和6个月时的μTBS低于某些含BAC的实验组（ p ＜0.05）。当BAC以0.5％和1.0％的浓度掺入粘合剂共混物中时，六个月后未观察到牙本质粘合强度的降低（p <0.05）。相比于DW预处理的对照组，BAC预处理的牙本质基质的质量损失和HYP释放更少（p <0.05）。这项体外研究表明，BAC通过减少胶原蛋白的降解有助于树脂-牙本质键的保持。
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