Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates

摘要

Production of extracellular chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 under solid substrate fermentation was studied. The suitability of shrimp shell chitin waste (SSCW) and commercial wheat bran (CWB) was evaluated for maximal enzyme production. CWB medium (pH 6.4 ± 0.2) supplemented with chitosan favoured maximal chitin deacetylase yield of 460.4 ± 14.7 unit/g initial dry substrate (U/g IDS) at 96 h as compared to maximal yield of 392.0 ± 6.4 U/g IDS at 192 h in SSCW medium (pH 8.7 ± 0.2) at 25 °C incubation temperature and 60% (w/w) initial moisture content of medium. Along with chitin deacetylase, C. lindemuthianum ATCC 56676 produced maximum endo-chitinase (0.28 ± 0.03 U/g IDS at 144 h) and β-N-acetylhexosaminidase (0.79 ± 0.009 U/g IDS at 192 h) in CWB medium and 0.49 ± 0.05 U/g IDS of endo-chitinase at 264 h and 0.38 ± 0.04 U/g IDS of β-N-acetylhexosaminidase at 96 h of incubation in SSCW medium. SEM studies indicated the difference in the morphology of mycelia and hyphae of C. lindemuthianum ATCC 56676 when grown on different solid substrates. Production of chitin deacetylase by SSF is being reported for the first time.