Fluorinated Methacrylamide Chitosan Sequesters Reactive OxygenSpecies to Relieve Oxidative Stress while Delivering Oxygen

摘要

Antioxidants play an important role in regulating overabundant reactive oxygen species (ROS) in wound healing to reduce oxidative stress and inflammation. In this work we demonstrate for the first time that functionalization of methacrylamide chitosan (MAC) with aliphatic pentadecafluoro chains, to synthesize pentadecafluoro-octanoyl methacrylamide chitosan (MACF), enhances the antioxidant capacity of the MAC base hydrogel material, while being able to deliver oxygen for enhanced wound healing. As such MACF was shown to sequester more nitric oxide (p < 0.01) and hydroxyl (p < 0.0001) radicals as compared to MAC and controls even when delivering additional oxygen. MACF’s beneficial antioxidant capacity was further confirmed in in vitro cell culture experiments using human dermal fibroblasts stressed with 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH).