Chemical characterization of Brickellia cavanillesii (Asteraceae) using gas chromatographic methods

摘要

A methanol extract of lyophilized Brickellia cavanillesii was quantitatively analyzed using gas chromatographic (GC) techniques. The chromatographic methods employed were (i) GC-flame ionization detector (GC-FID), (ii) GC-mass spectrometry (GC-MS), and (iii) purge and trap GC-MS (P&T GC-MS). Thirteen compounds were identified with a quality match of 90% and above using GC-MS. The compounds were (>1) Cyclohexene, 6-ethenyl-6-methyl-1-(1-methylethyl)-3-(1-methylethylidene)-, (S)-; (>2) Bicylo (2.2.1) heptan-2-one, 1, 7, 7-trimethyl-(1S, 4S)-; (>3) Phenol, 2-methoxy-4-(1-propenyl)-; (>4) Benzene, 1-(1, 5-dimethyl-4-hexenyl)-4-methyl-; (>5) Naphthalene, 1, 2, 3, 5, 6, 8a-hexahydro4, 7-dimethyl-1-1-(1-methylethyl)-, (1S-cis)-; (>6) Phenol, 2-methoxy-; (>7) Benzaldehyde, 3-hydroxy-4-methoxy-; (>8) 11, 13-Eicosadienoic acid, methyl ester; (>9) 2-Furancarboxaldehyde, 5-methyl-; (>10) Maltol; (>11) Phenol; (>12) Hydroquinone; (>13) 1H-Indene, 1-ethylideneoctahydro-7a-methyl-, (1E, 3a.alpha, 7a.beta.). Other compounds (>14) 3-methyl butanal; (>15) (D)-Limonene; (>16) 1-methyl-4-(1-methyl ethyl) benzene; (>17) Butanoic acid methyl ester; (>18) 2-methyl propanal; (>19) 2-butanone; (>20) 2-pentanone; and (>21) 2-methyl butane were also identified when P&T GC-MS was performed. Of the 21 compounds identified, 12 were validated using chemical standards. The identified compounds were found to be terpenes, derivatives of terpenes, esters, ketones, aldehydes, and phenol-derived aromatic compounds; these are the primary constituents of the essential oils of many plants and flowers.