Enzymatic hydrolysis of cassava peels was evaluated using cellulase and beta-glucanase enzymes and their mixtures at three different enzyme loadings with time. The pH of the medium used for hydrolysis was 5 and the temperature was 50 °C. The efficiency of the hydrolysis using beta-glucanase was better than cellulase and glucose recovery of 69 % was realised when beta-glucanase dosage was increased to 10 % (v/w) at 48 h which rose to 73 % at 120 h, releasing 11.19 g/l and 12.17 g/l of glucose respectively. Less than 20 % of glucose was hydrolysed at 10 % (v/w) cellulase at 120 h releasing 2.6 g/l glucose. The optimum experimental condition for hydrolysis of cassava peel was established at 120 h when glucose recovery increased to 88 % for enzyme mixture of 5 % (v/w) cellulase + 10 % (v/w) beta-glucanase producing 14.67 g/l glucose in the hydrolysate.
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机译:使用纤维素酶和β-葡聚糖酶及其混合物在三种不同的酶负载下随时间评估木薯皮的酶促水解。用于水解的介质的pH值为5,温度为50°C。使用β-葡聚糖酶的水解效率要好于纤维素酶,当β-葡聚糖酶的剂量在48小时时增加至10%(v / w),在120小时时上升至73%,释放11.19时,葡萄糖的回收率达到69%。 g / l和12.17 g / l的葡萄糖。在120小时以10%(v / w)的纤维素酶水解不到20%的葡萄糖,释放出2.6 g / l的葡萄糖。木薯果皮水解的最佳实验条件是在120h时建立的,当5%(v / w)纤维素酶+ 10%(v / w)β-葡聚糖酶的酶混合物的葡萄糖回收率提高到88%时,可产生14.67 g / l葡萄糖在水解产物中。
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