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Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress

机译:干旱胁迫下天竺葵差异表达基因的全转录组序列分析

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摘要

Phormium tenax is a kind of drought resistant garden plant with its rich and colorful leaves. To clarify the molecular mechanism of drought resistance in Phormium tenax, transcriptome was sequenced by the Illumina sequencing technology under normal and drought stress, respectively. A large number of contigs, transcripts and unigenes were obtained. Among them, only 30,814 unigenes were annotated by comparing with the protein databases. A total of 4,380 genes were differentially expressed, 2,698 of which were finally annotated under drought stress. Differentially expression analysis was also performed upon drought treatment. In KEGG pathway, the mechanism of drought resistance in Phormium tenax was explained from three aspects of metabolism and signaling of hormones, osmotic adjustment and reactive oxygen species metabolism. These results are helpful to understand the drought tolerance mechanism of Phormium tenax and will provide a precious genetic resource for drought-resistant vegetation breeding and research.
机译:Tenax is是一种抗旱的园林植物,叶子丰富而多彩。为了阐明ten虫抗旱的分子机制,分别通过Illumina测序技术对转录组进行了测序,分别在正常和干旱胁迫下进行。获得了大量的重叠群,转录本和单基因。其中,通过与蛋白质数据库比较,仅注释了30,814个单基因。共有4,380个基因被差异表达,其中2,698个基因最终在干旱胁迫下被注释。干旱处理后也进行了差异表达分析。在KEGG途径中,从激素的代谢和信号传导,渗透调节和活性氧代谢三个方面解释了ten的抗旱机理。这些结果有助于了解ten虫的抗旱机理,为抗旱植被的育种和研究提供宝贵的遗传资源。

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