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Kermit: linkage map guided long read assembly

机译:柯米特(Kermit):链接图指导的长读汇编

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摘要

Background With long reads getting even longer and cheaper, large scale sequencing projects can be accomplished without short reads at an affordable cost. Due to the high error rates and less mature tools, de novo assembly of long reads is still challenging and often results in a large collection of contigs. Dense linkage maps are collections of markers whose location on the genome is approximately known. Therefore they provide long range information that has the potential to greatly aid in de novo assembly. Previously linkage maps have been used to detect misassemblies and to manually order contigs. However, no fully automated tools exist to incorporate linkage maps in assembly but instead large amounts of manual labour is needed to order the contigs into chromosomes.
机译:背景技术随着长读变得更长,更便宜,可以完成大规模测序项目,而无需短读,价格可承受。由于较高的错误率和较不成熟的工具,长读的从头组装仍然具有挑战性,通常会导致大量重叠群。密集的连锁图谱是其在基因组上的位置大约已知的标记物的集合。因此,它们提供的远程信息有可能极大地帮助从头组装。以前,链接图已用于检测组装错误和手动订购重叠群。但是,不存在将装配图整合到装配中的全自动工具,而是需要大量的人工操作才能将重叠群定为染色体。

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