The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found in total bacterial numbers either by roll tube or most-probable-number procedures when lyophilized rumen fluid residue was substituted for fresh rumen fluid. Lyophilized rumen fluid residue was stable for at least 5 months at room temperature. Rumen fluid supernatant from centrifugation at 1,000 x g had a mean density of 1.005 +/- 0.03 g/ml and contained 1.56% +/- 0.30% dry matter. On the basis of these values, 15.68 mg of lyophilized rumen fluid residue is equivalent to 1 ml of rumen fluid supernatant from centrifugation at 1,000 x g.
展开▼
机译：将在1,000 x g的过滤过的瘤胃液中离心分离的上清液冻干，并使用残留物和升华级分代替完整的卷管培养基中的新鲜瘤胃液，以计数总瘤胃细菌。新鲜的瘤胃液中大多数支持生长的营养物质都存在于残留物中。除了一个例外，当用冻干的瘤胃液残留物代替新鲜瘤胃液时，通过滚管法或最可能次数的程序在总细菌数上没有发现显着差异。冻干的瘤胃液残留物在室温下稳定至少5个月。以1,000×g离心得到的瘤胃液上清液的平均密度为1.005 +/- 0.03g / ml，并且含有1.56％+ /-0.30％的干物质。基于这些值，以1,000 x g离心分离得到的15.68 mg冻干瘤胃液上清液相当于1 ml瘤胃液上清液。
展开▼
