Method for producing porifera spicule powder through impregnation lyophilization coating of human umbilical cord blood derived cell culture fluid and cosmetic composition using the same and method for producing the cosmetic

摘要

The present invention relates to a method for manufacturing a sponge powder through an impregnated freeze-dried coating of a human umbilical cord blood cell culture solution, and a cosmetic composition and a cosmetic manufacturing method using the same. In the present invention, the step of preparing a predetermined amount of the needle-shaped sponge bone fragment powder having the porosity secured by the high-pressure enzyme digestion method, which is a non-chemically active method; Dissolving the human cord blood cell culture solution USC1994-FP powder in a solvent; Preparing pressure and temperature conditions of an air mixer; Injecting a predetermined amount of needle-shaped spongy bone fragment powder and a predetermined amount of human umbilical cord blood cell culture solution dissolved by a solvent into the air mixer; Mixing the needle-shaped sponge powder and the human umbilical cord blood cell culture liquid dissolved by the solvent in the air mixer with compressed air, and coating and impregnating the human umbilical cord blood cell culture liquid on the needle-shaped sponge bone fragments; Freeze-drying in the air mixer in a state in which the powder of the needle-shaped sponge fragment and the human umbilical cord blood cell culture solution are mixed; A method of preparing a needle-shaped sponge bone powder through an impregnation freeze-dried coating of a human umbilical cord blood cell culture liquid comprising the step of obtaining a freeze-dried powder by coating and impregnating the powder of the needle-shaped sponge fragment and the human umbilical cord blood cell culture liquid, using the same A cosmetic composition and a method of manufacturing a cosmetic are presented.