Molecular cloning expression and characterization of four novel thermo-alkaliphilic enzymes retrieved from a metagenomic library

摘要

BackgroundEnzyme discovery is a promising approach to aid in the deconstruction of recalcitrant plant biomass in an industrial process. Novel enzymes can be readily discovered by applying metagenomics on whole microbiomes. Our goal was to select, examine, and characterize eight novel glycoside hydrolases that were previously detected in metagenomic libraries, to serve biotechnological applications with high performance.