Prediction of RNA-binding amino acids from protein and RNA sequences

摘要

BackgroundMany learning approaches to predicting RNA-binding residues in a protein sequence construct a non-redundant training dataset based on the sequence similarity. The sequence similarity-based method either takes a whole sequence or discards it for a training dataset. However, similar sequences or even identical sequences can have different interaction sites depending on their interaction partners, and this information is lost when the sequences are removed. Furthermore, a training dataset constructed by the sequence similarity-based method may contain redundant data when the remaining sequence contains similar subsequences within the sequence. In addition to the problem with the training dataset, most approaches do not consider the interacting partner (i.e., RNA) of a protein when they predict RNA-binding amino acids. Thus, they always predict the same RNA-binding sites for a given protein sequence even if the protein binds to different RNA molecules.