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Efficacy of one dose vaccination against experimental infection with two Mycoplasma hyopneumoniae strains

机译:一剂疫苗对两种猪肺炎支原体菌株的实验感染的功效

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摘要

Background Mycoplasma hyopneumoniae (M. hyopneumoniae) is the primary agent of enzootic pneumonia in pigs. Pigs are often infected with different M. hyopneumoniae strains. This study assessed the efficacy of vaccination against experimental infection with two genetically different M. hyopneumoniae strains in weaned piglets. At 33 days of age (D0), 45 M. hyopneumoniae-free piglets were randomly assigned to three different groups: 1) negative control group (NCG; n = 5): not vaccinated, not infected, 2) positive control group (PCG; n = 20): not vaccinated, infected, and 3) vaccination group (VG; n = 20): single vaccination with an inactivated whole-cell M. hyopneumoniae vaccine (Hyogen®, Ceva) (D1), infected. The PCG and VG were endotracheally inoculated with 7 × 107 CCU in 7 ml of the highly virulent M. hyopneumoniae strain F7.2C (D24) and 7 × 107 CCU in 7 ml low virulent strain F1.12A (D25). A respiratory disease score (RDS) was assessed from D24 until D53. At D53 (euthanasia), macroscopic lung lesions (MLL) were scored, log copies of M. hyopneumoniae DNA (qPCR) and IL-1 and IL-6-concentrations (ELISA) on bronchoalveolar lavage fluid were determined.
机译:背景猪肺炎支原体(M. hyopneumoniae)是猪内生性肺炎的主要病原。猪经常感染不同的猪肺炎支原体菌株。这项研究评估了断奶仔猪中两种遗传上不同的猪肺炎支原体菌株疫苗接种对实验性感染的功效。在第33天(D0),将45只无猪肺炎支原体的仔猪随机分为三个不同的组:1)阴性对照组(NCG; n = 5):未接种疫苗,未感染,2)阳性对照组(PCG) ; n = 20):未接种,未感染,3)疫苗接种组(VG; n = 20):灭活的全细胞猪肺炎支原体疫苗(Hyogen®,Ceva)(D1)的单次疫苗接种。气管内接种7 ml高毒力肺炎支原体F7.2C(D24)和7×10 7 CCU中的7×10 7 CCU加入7毫升低毒力菌株F1.12A(D25)。从D24到D53评估呼吸道疾病评分(RDS)。在D53(安乐死),对肺部宏观病变(MLL)进行评分,确定猪肺炎支原体DNA(qPCR)的对数副本以及支气管肺泡灌洗液中IL-1和IL-6浓度(ELISA)。

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