2010 NS-AUA Abstracts

摘要

Background:Urinary tract infections (UTI) are among the most common bacterial infections in the United States. The gold standard for diagnosis is urine culture. This provides bacteria identification and sensitivity, but is labor intensive and time-consuming. As such, many UTIs are treated empirically contributing to resistance and cost. There is an urgent need for a diagnostic tool that can rapidly identify uropathogens and their sensitivities. We present the development of a PCR-based assay for Enterobacter cloacae, and evidence for its further use in the context of NanoLantern™ technology. The NanoLantern™ is a chip sensor that utilizes DNA hairpins immobilized onto a gold surface. In the presence of complementary (target) DNA, the hairpin stem is opened, exposing a quantifiable fluorophore.