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Is the Goat a New Host for the G3 Indian Buffalo Strain of Echinococcus granulosus?

机译:山羊是G3印度水牛细粒棘球oc菌株的新寄主吗?

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摘要

Four goats bred in Central Italy (province of Rieti) revealed, in the liver, metacestodes of Echinococcus granulosus. The cysts, unilocular and fertile, were examined by microscopy and molecular diagnostics. Morphological data on the rostellar hooks are in agreement with the original description of the strain found in buffaloes and are largely compatible with those reported in Europe for cattle and humans. Specific PCR followed by DNA sequencing of the mitochondrial cox1 gene revealed for all the isolates 99.5% identity to the reference strain G3 genotype and 99.3% and 99.1% to G2 and G1, respectively. Further genetic markers (nad1 and 12S rRNA) confirmed the identity of the goat isolates to the G3 strain. This genotype, here reported for the first time in goats, proved to have a wider than previously supposed host range, therefore its relevance in human hydatidosis is expected to be more often evidenced.
机译:在意大利中部(列蒂省)饲养的四只山羊在肝脏中发现了细粒棘球oc虫的后代。通过显微镜检查和分子诊断检查了单眼和可育的囊肿。十字形钩上的形态学数据与在水牛中发现的菌株的原始描述相符,并且与欧洲报道的有关牛和人的菌株基本相符。特异性PCR,然后对线粒体cox1基因进行DNA测序,发现所有分离株与参考菌株G3基因型的同源性为99.5%,与G2和G1的同源性分别为99.3%和99.1%。进一步的遗传标记(nad1和12S rRNA)证实了山羊分离株与G3株的身份相同。该基因型首次在山羊中报道,被证明具有比以前认为的宿主范围更广的基因型,因此,有望进一步证实其与人类hy虫症的相关性。

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