首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Unidirectional Brownian motion observed in an in silico single molecule experiment of an actomyosin motor
【2h】

Unidirectional Brownian motion observed in an in silico single molecule experiment of an actomyosin motor

机译:在肌动球蛋白马达的计算机单分子实验中观察到单向布朗运动

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。
获取外文期刊封面目录资料

摘要

The actomyosin molecular motor, the motor composed of myosin II and actin filament, is responsible for muscle contraction, converting chemical energy into mechanical work. Although recent single molecule and structural studies have shed new light on the energy-converting mechanism, the physical basis of the molecular-level mechanism remains unclear because of the experimental limitations. To provide a clue to resolve the controversy between the lever-arm mechanism and the Brownian ratchet-like mechanism, we here report an in silico single molecule experiment of an actomyosin motor. When we placed myosin on an actin filament and allowed myosin to move along the filament, we found that myosin exhibits a unidirectional Brownian motion along the filament. This unidirectionality was found to arise from the combination of a nonequilibrium condition realized by coupling to the ATP hydrolysis and a ratchet-like energy landscape inherent in the actin-myosin interaction along the filament, indicating that a Brownian ratchet-like mechanism contributes substantially to the energy conversion of this molecular motor.
机译:肌动球蛋白分子马达是由肌球蛋白II和肌动蛋白丝组成的马达,负责肌肉收缩,将化学能转化为机械功。尽管最近的单分子和结构研究为能量转换机理提供了新的思路,但是由于实验的局限性,分子水平机理的物理基础仍然不清楚。为了提供解决杠杆臂机制与布朗氏棘轮状机制之间争议的线索,我们在此报告了一种肌动球蛋白马达的计算机单分子实验。当我们将肌球蛋白放在肌动蛋白丝上并使肌球蛋白沿着丝运动时,我们发现肌球蛋白沿丝表现出单向布朗运动。发现这种单向性是由于结合到ATP水解而实现的非平衡条件与沿丝的肌动蛋白-肌球蛋白相互作用中固有的棘轮状能量态的结合而产生的,这表明布朗的棘轮状机制对促成分子马达的能量转换。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号