首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Biochemical and Genetic Studies of Recombination Proficiency in Escherichia coli I. Enzymatic Activity Associated with recB+ and recC+ Genes
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Biochemical and Genetic Studies of Recombination Proficiency in Escherichia coli I. Enzymatic Activity Associated with recB+ and recC+ Genes

机译:大肠杆菌重组能力的生化和遗传研究I。与recB +和recC +基因相关的酶活性

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摘要

An adenosine triphosphate-dependent deoxyribonuclease activity has been detected in lysates of recB+ recC+ strains. Mutations in recB or recC lead to loss of this activity, suggesting that these two genes determine the nuclease activity. The over-all reaction in crude lysates digests native DNA to nucleoside monophosphates. Complementation between recB21 and recC22 in vivo leads to normal levels of ATP-dependent nuclease activity. No complementation in vitro has been detected. Mutations in a third recombination gene (recA) do not alter significantly the wild-type levels of this nuclease activity.
机译:在recB + recC + 菌株的裂解物中检测到三磷酸腺苷依赖性的脱氧核糖核酸酶活性。 recB或recC中的突变导致该活性的丧失,表明这两个基因决定了核酸酶的活性。粗裂解物中的总体反应将天然DNA消化成核苷一磷酸。体内recB21和recC22之间的互补导致正常水平的ATP依赖性核酸酶活性。没有检测到体外互补。第三重组基因(recA)中的突变不会显着改变该核酸酶活性的野生型水平。

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