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Antioxidant and Anti-Inflammatory Effects of Chaenomeles sinensis Leaf Extracts on LPS-Stimulated RAW 264.7 Cells

机译:木瓜叶提取物对LPS刺激的RAW 264.7细胞的抗氧化和抗炎作用

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摘要

The fruit of Chaenomeles sinensis has been traditionally used in ethnomedicine for the treatment of various human ailments, including pneumonia, bronchitis, and so on, but the pharmacological applications of the leaf part of the plant have not been studied. In this study, we evaluated the various radical scavenging activities and anti-inflammatory effects of different Chaenomeles sinensis leaf (CSL) extracts. The water extract showed a higher antioxidant and radical scavenging activities. However the ethanolic extracts showed higher NO scavenging activity than water extract, therefore the ethanolic extract of CSL was examined for anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The 70% ethanol extract of CSL (CSLE) has higher anti-inflammatory activity and significantly inhibited the production of nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). In addition, CSLE suppressed LPS-stimulated inducible nitric oxide synthase (iNOS) and NO production, IL-1β and phospho-STAT1 expression. In this study, we investigated the effect of CSLE on the production of inflammatory mediators through the inhibition of the TRIF-dependent pathways. Furthermore, we evaluated the role of CSLE on LPS-induced expression of pro-inflammatory cytokines, such as TNF-α, IL-1β and IL-6. Our results suggest that CSLE attenuates the LPS-stimulated inflammatory responses in macrophages through regulating the key inflammatory mechanisms, providing scientific support for its traditional uses in treating various inflammatory diseases.
机译:木瓜木瓜的果实传统上已被用于民族药中,用于治疗各种人类疾病,包括肺炎,支气管炎等,但是尚未对该植物叶部的药理应用进行研究。在这项研究中,我们评估了不同的木瓜叶(CSL)提取物的各种自由基清除活性和抗炎作用。水提取物显示出较高的抗氧化剂和自由基清除活性。然而,乙醇提取物显示出比水提取物更高的NO清除活性,因此检查了CSL乙醇提取物对脂多糖(LPS)刺激的RAW 264.7细胞的抗炎作用。 CSL(CSLE)的70%乙醇提取物具有较高的抗炎活性,并显着抑制一氧化氮(NO),白介素6(IL-6)和肿瘤坏死因子-α(TNF-α)的产生。此外,CSLE抑制LPS刺激的诱导型一氧化氮合酶(iNOS)和NO的产生,IL-1β和磷酸STAT1的表达。在这项研究中,我们研究了CSLE通过抑制TRIF依赖性途径对炎性介质产生的影响。此外,我们评估了CSLE在LPS诱导的促炎性细胞因子(如TNF-α,IL-1β和IL-6)表达中的作用。我们的结果表明,CSLE通过调节关键的炎症机制来减弱巨噬细胞中LPS刺激的炎症反应,为其在各种炎症疾病中的传统用途提供科学支持。

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