Determination of squalene in edible oils by transmethylation and GC analysis

摘要

class="kwd-title">Method name: Squalene determination by GCFID class="kwd-title">Keywords: Fatty acid methyl esters, Basic catalyst, 23:0, Internal standard class="head no_bottom_margin" id="abs0010title">AbstractSqualene is a triterpenoid with medicinal, therapeutic and cosmetic applications. There is interest in identifying novel sources of squalene, creating a need for a rapid and accurate method of squalene determination. Here, we describe such an approach that involves first transmethylating the oil base containing squalene using a basic catalyst, followed by addition of internal standard and quantification by gas chromatography flame ionization detection (GCIFD). class="first-line-outdent" id="lis0005">

• The method uses a single reference standard for quantification.

• Validation showed it to be both repeatable and accurate.

• It was successfully applied to nutraceutical and edible oils containing squalene.