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Molecular Screening of Blast Resistance Genes in Rice using SSR Markers

机译:利用SSR标记分子筛查稻瘟病抗性基因

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摘要

Rice Blast is the most devastating disease causing major yield losses in every year worldwide. It had been proved that using resistant rice varieties would be the most effective way to control this disease. Molecular screening and genetic diversities of major rice blast resistance genes were determined in 192 rice germplasm accessions using simple sequence repeat (SSR) markers. The genetic frequencies of the 10 major rice blast resistance genes varied from 19.79% to 54.69%. Seven accessions IC337593, IC346002, IC346004, IC346813, IC356117, IC356422 and IC383441 had maximum eight blast resistance gene, while FR13B, Hourakani, Kala Rata 1–24, Lemont, Brown Gora, IR87756-20-2-2-3, IC282418, IC356419, PKSLGR-1 and PKSLGR-39 had seven blast resistance genes. Twenty accessions possessed six genes, 36 accessions had five genes, 41 accessions had four genes, 38 accessions had three genes, 26 accessions had two genes, 13 accessions had single R gene and only one accession IC438644 does not possess any one blast resistant gene. Out of 192 accessions only 17 accessions harboured 7 to 8 blast resistance genes.
机译:稻瘟病是最毁灭性的疾病,每年在全世界范围内都会导致大量减产。已经证明,使用抗性水稻品种将是控制这种疾病的最有效方法。使用简单序列重复(SSR)标记对192种水稻种质中主要稻瘟病抗性基因的分子筛选和遗传多样性进行了测定。 10个主要的稻瘟病抗性基因的遗传频率在19.79%至54.69%之间。七个登录号IC337593,IC346002,IC346004,IC346813,IC356117,IC356422和IC383441具有最大的八个抗瘟基因,而FR13B,Hourakani,Kala Rata 1-24,Lemont,Brown Gora,IR87756-20-2-2-3,IC282418, IC356419,PKSLGR-1和PKSLGR-39具有七个抗稻瘟病基因。 20份具有6个基因,36份具有5个基因,41份具有4个基因,38份具有3个基因,26份具有2个基因,13份具有单个R基因,只有1个IC438644不具有任何抗病基因。在192个种质中,只有17个种质具有7至8个抗药性基因。

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