We have isolated and characterized a human cDNA (HBK2) that is homologous to novel member (RCK2) of the K+ channel RCK gene family expressed in rat brain. RCK2 mRNA was detected predominantly in midbrain areas and brainstem. The primary sequences of the HBK2/RCK2 K+ channel proteins exhibit major differences to other members of the RCK gene family. The bend region between segments S1 and S2 is unusually long and does not contain the N-glycosylation site commonly found in this region. They might be O-glycosylated instead. Functional characterization of the HBK2/RCK2 K+ channels in Xenopus laevis oocytes following micro-injection in in vitro transcribed HBK2 or RCK2 cRNA showed that the HBK2/RCK2 proteins form voltage-gated K+ channels with novel functional and pharmacological properties. These channels are different to RCK1, RCK3, RCK4 and RCK5 K+ channels.
展开▼
机译：我们已经分离和表征了与在大鼠脑中表达的K +通道RCK基因家族的新成员（RCK2）同源的人cDNA（HBK2）。 RCK2 mRNA主要在中脑区域和脑干中检测到。 HBK2 / RCK2 K +通道蛋白的主要序列与RCK基因家族的其他成员存在主要差异。片段S1和S2之间的弯曲区域异常长，并且不包含该区域中常见的N-糖基化位点。它们可能被O-糖基化。在体外转录的HBK2或RCK2 cRNA微注射后，非洲爪蟾卵母细胞中HBK2 / RCK2 K +通道的功能表征显示，HBK2 / RCK2蛋白形成具有新功能和药理特性的电压门控K +通道。这些通道不同于RCK1，RCK3，RCK4和RCK5 K +通道。
展开▼
