Proton pump inhibitors can reverse the YAP mediated paclitaxel resistance in epithelial ovarian cancer

摘要

YAP is highly expressed in EOC and is directly regulated by V-ATPase D1 expression. Immunohistochemical observation of V-ATPase D1 and YAP protein expression in normal ovarian epithelium and EOC tissues, V-ATPase D1 and YAP (with extremely weak staining (0), a weak staining (+ 1), a moderate staining (+ 2), a strong staining (+ 3). All photographs were taken at original 200 × magnification. The scores of ( ) V-ATPase D1 and ( ) YAP by beeswarm plot was shown in the bottom (**  c The mRNA level of V-ATPase D1 and YAP in normal ovarian epithelium and EOC tissues were determined using a real-time PCR kit. Data represent the mean and standard deviation (mean ± SD) are from three independent experiments (*  d V-ATPase D1 and YAP expression assessed by Q-PCR analysis in EOC cell lines. Data (mean ± SD) are from three independent experiments (*  e V-ATPase D1, YAP and p-YAP protein levels in A2780/T cells were performed by western blot. Knocking down the expression of V-ATPase D1 in A2780/T cells impaired the transcriptional activity of YAP in the luciferase assay. Knocking down the expression of V-ATPase D1 in A2780/T cells impaired the binding of YAP to the promoter region of CTGF and Cyr61