Substantial interassay variability (up to 20%) has been described for vancomycin immunoassays in adults, but the impact of neonatal matrix is difficult to quantify because of blood volume constraints in neonates. However, we provide circumstantial evidence for a similar extent of variability. Using the same vancomycin dosing regimens and confirming similarity in clinical characteristics, vancomycin trough concentrations measured by PETINIA (2011-2012, n = 400) were 20% lower and the mean difference was 1.93 mg/L compared to COBAS (2012–2014, n = 352) measurements. The impact of vancomycin immunoassays in neonatal matrix was hereby suggested, supporting a switch to more advanced techniques (LC-MS/MS).
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机译：对于成人万古霉素免疫测定，已有大量的测定间差异（高达20％），但由于新生儿的血容量受限，难以量化新生儿基质的影响。但是，我们为相似程度的可变性提供了间接证据。使用相同的万古霉素给药方案并确认临床特征相似，PETINIA（2011-2012，n = 400）测量的万古霉素谷浓度比COBAS（2012-2014，n）低20％，平均差异为1.93μmg/ L = 352）测量值。因此，建议使用万古霉素免疫测定法对新生儿基质的影响，这支持转向更先进的技术（LC-MS / MS）。
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