脂氧素A4受体ALXR在子宫内膜异位症患者在位及异位子宫内膜组织中的表达及意义

摘要

Objective To investigate the expression of lipoxin A4 receptor (ALXR) in eutopic and ectopic endometrium of patients with endometriosis. Methods Twenty seven patients with endometriosis underwent laparoscopic surgery in our hospital between February 2012 and September 2012, the tissue samples of eutopic and ectopic endometrium were col ected;and uterine endometrium samples were also col ected from 30 patients with benign ovary cysts as controls. The mRNA and protein expression of ALXR in the eutopic endometrium, ectopic endometrium of endometriosis patients and control endometri-um were detected by real- time fluorescent quantitative RT- PCR and immunohistochemistry, respectively. Results The mRNA expression of ALXR was 0.069 ±0.013 in ectopic endometrium and 0.039 ±0.013 in eutopic endometrium of endometriosis, which were significantly higher than that of the control (0.019±0.008, P<0.05). The mRNA expression of ALXR in the ectopic endometrium was also higher than that of eutopic endometrium of endometriosis (P<0.05). Compared with the control, the ex-pression of ALXR protein in eutopic and ectopic endometrium of endometriosis were increased (P<0.05). Conclusion The ec-topic and eutopic endometrium in patients with endometriosis shows high expression of lipoxin A4 receptor mRNA and protein, which indicates that ALXR may be associated with the pathogenesis of endometriosis.%目的：探讨脂氧素A4受体ALXR在子宫内膜异位症（EMs）患者在位及异位内膜组织中的表达水平及其临床意义。方法因EMs行腹腔镜手术治疗的患者27例，留取在位子宫内膜组织（EMs在位内膜组）和异位子宫内膜组织（EMs异位内膜组），取同期因良性卵巢囊肿行腹腔镜手术治疗患者30例为对照组，留取宫腔内正常内膜组织。分别采用实时荧光定量PCR技术、免疫组化法检测各组内膜组织中ALXR mRNA及蛋白表达水平。结果 EMs异位内膜组ALXR mRNA表达水平为0.069±0.020，EMs在位内膜组为0.039±0.013，对照组为0.019±0.008,EMs异位和在位内膜组的表达水平均明显高于对照组（均P＜0.05），EMs异位内膜组又高于在位内膜组（P＜0.05）。EMs异位和在位内膜组ALXR蛋白表达水平明显高于对照组（P＜0.05）。结论 ALXR mRNA及蛋白在EMs异位及在位内膜组织中均呈高表达状态，推测ALXR可能与EMs发病有关。