Objective To evaluate single detection of Golgi protein 73(GP73) or combined with other serum tumor markers in diagnosis of HBSAg- positive hepatocellular carcinoma (HCC). Methods Serum samples were col ected from 133 cases of HBsAg- positive HCC, 85 cases of chronic hepatitis B (CHB), 74 cases of liver cirrhosis (LC) infected with HBV and 46 healthy subjects. Serum levels of GP73, AFP, CEA, SF, CA19- 9, CA15- 3, CA125, ALP, GGT and LAP were assayed and the results were analyzed. Results Spearman correlation analysis showed that levels of serum GP73 was associated with the levels of AFP, CEA, SF, CA19- 9, CA15- 3, CA125, ALP, GGT and LAP (P<0.05), not with CA125;the correlation coefficient of AFP, GGT and LAP in HCC group were lower than that in non- HCC group (P<0.05). There were significant differences in serum levels of GP73, AFP, CEA, SF, CA19- 9, CA15- 3, CA125, ALP, GGT and LAP between CHB, LC, HCC patients and normal controls (P<0.05). The serum levels of GP73, AFP and LAP in HCC group were significantly higher than those in other groups(P<0.05). ROC curve anal-ysis showed that there was no significant difference in area under ROC among GP73, AFP and ALP (Z=0.127 and 1.908 respec-tively, P=0.899 and 0.056 respectively), although the AUC of GP73 was the largest (AUC=0.792). By logistic regression, we used all these indicators to build a new prediction model. The results showed that the AUC of the combined markers was significantly higher than any single markers (P<0.05). Conclusion GP73 is a high sensitive and specific serum mark for diagnosis of PHC and the detection of GP73 combined with AFP, CEA, SF, CA19- 9, CA15- 3, CA125, ALP, GGT and LAP can improve the diag-nostic value for HBsAg- positive HCC patients.%目的探讨血清高尔基体蛋白73（GP73）及甲胎蛋白（AFP）、癌胚抗原（CEA）、铁蛋白（SF）、糖基抗原（CA19-9、CA15-3、CA125）、碱性磷酸酶（ALP）、γ-谷氨酰基转肽酶（GGT）、亮氨酸氨基转肽酶（LAP）等肿瘤标志物单项和联合检测对乙型肝炎（乙肝）病毒表面抗原（HBsAg）阳性肝癌的诊断价值。方法收集85例慢性乙肝、74例乙肝肝硬化、133例HBsAg阳性肝癌患者及46例健康体检者的血清，对GP73及AFP、CEA、SF、CA19-9、CA15-3、CA125、ALP、GGT、LAP等肿瘤标志物进行检测，并对检测结果进行统计学分析。结果 Spearman相关分析表明血清GP73与AFP、CEA、SF、CA19-9、CA15-3、CA125、ALP、GGT、LAP均有一定的相关性（均P＜0.05），除CA125外，肝癌组的相关系数均比非肝癌组小；但只有GP73与AFP、GGT及LAP的相关性在两组间的差异有统计学意义（均P＜0.05）。GP73、AFP、CEA、SF、CA19-9、CA15-3、CA125、ALP、GGT、LAP在慢性乙肝组、乙肝肝硬化组、肝癌组和正常对照组中的差异均有统计学意义（均P＜0.05），肝癌组的GP73、AFP、LAP的血清水平均显著高于其他组（均P＜0.05）。ROC曲线分析表明，单项指标中以GP73的ROC曲线下面积最大（AUC=0.792）,但与AFP和ALP的差异不大（Z=0.127、1.908，P=0.899、0.056）。通过logistic回归对上述指标构建新的预测模型，联合预测因子（PRE）的AUC显著高于其它单项指标（均P＜0.05）。结论 GP73单项检测有较好的诊断作用，GP73联合AFP、CEA、SF、CA19-9、CA15-3、CA125、ALP、GGT、LAP检测可提高对HBsAg阳性肝癌的诊断效能。
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