Objective To explore the difference of the expression of ribosomal protein S15a RPS15a is the gastric carcinoma and paracancerous tissue, for better understanding the molecular mechanism of the carcinogenesis and progress of gastric carcinoma. Method Fluorescent quantitive PCR was applied to test the difference of the gene expression in the gastric carcinoma and paracancerous tissue, which were compared with the its expression in the various types to tumor cells. Result RPS15a was highly expressed in the gastric carcinoma, indicating its relationship with the malignant biological hehavior of the tumor cells. The espressions were not significantly different among various types of tumor cells, which implies than such expression may be universal in malignant tumors.%目的：研究核糖体蛋白S15a（ribosomal protein S15a RPS15a）基因在胃癌及癌旁组织中表达差异，为进一步了解胃癌发生、发展的分子机制提供帮助。方法应用荧光定量PCR等方法进一步验证该基因在胃癌及癌旁组织中的表达差异，并在多种肿瘤细胞中的表达量进行比较。结果该基因在胃癌组织中的表达量高于其对应的癌旁组织，但在多种肿瘤细胞中的表达量无显著差异。结论 RPS15a基因在胃癌中呈高表达，说明其可能与细胞恶性生物学行为相关。在多种肿瘤细胞中表达量无显著差异，推测其可能在恶性肿瘤中的高表达具有普遍性。
展开▼
