组合培养下Hep-2对EPCs的影响

摘要

Aim In the present study,laryngeal squamous cell carcinoma cells were co-cultured with endothelial progenitor cells in transwell chamber experiments to investigate the infl uence of co-culture on both cell types.MethodsAfter co-culturing,proliferation,apoptosis,cell cycle and tube formation by endothelial progenitor cells were measured. Changes in expression of cell surface markers and Fascin-1 were detected by immunocytochemistry. ResultsBoth the proliferation and apoptosis indices increased in endothelial progenitor cells and laryngeal squamous cell carcinoma cells after co-culture. Co-culturing upregulated the expressions of endothelial progenitor cells surface markers. Fascin-1 expression in laryngeal squamous cell carcinoma cells was increased and the angiogenic capacity of EPCs was enhanced.ConclusionCo-culture of endothelial progenitor cells with laryngeal squamous cell carcinoma cells induces changes in biological characteristics and angiogenic capacity,thereby promoting tumor angiogenesis.%目的：探讨喉鳞癌细胞与内皮祖细胞组合培养后对两种细胞的影响。方法在组合培养后，观察内皮祖细胞的细胞增殖、凋亡、细胞周期以及成血管能力变化。通过免疫组化检测细胞表面分子标注物的变化。结果在与喉鳞癌细胞组合培养后，内皮祖细胞的细胞增殖和凋亡均增加，上调细胞表面标志物。喉鳞癌细胞Fascin-1表达上调，内皮祖细胞的成血管能力加强。结论内皮祖细胞与喉鳞癌细胞组合培养可以导致细胞生物学特性和成血管能力的变化，从而促进肿瘤血管生成。