CyclinD1和CDC25A 在胆囊癌中的表达及意义

摘要

目的：通过检测CyclinD1和CDC25A在胆囊癌中的表达，探讨其在胆囊癌发生发展中的作用及其机制，为胆囊癌诊断和治疗寻找新的靶点。方法采用流式细胞术检测44例胆囊癌和35例慢性胆囊炎黏膜组织的CyclinD1、CDC25A蛋白的表达，其中蛋白的表达量用平均荧光强度（均道值）表示。结果胆囊癌组织、慢性胆囊炎黏膜组织中CyclinD1蛋白表达分别为（531.153±28.823）、（496.462±37.191），胆囊癌组织的CyclinD1蛋白表达高于正常组织；胆囊癌组织、慢性胆囊炎黏膜组织中CDC25A蛋白表达分别为（535.029±35.647）、（496.267±41.345），胆囊癌组织的CDC25A蛋白表达高于慢性炎症组织。结论 CyclinD1和CDC25A蛋白在胆囊癌组织中均高表达。这两种基因和蛋白表达的改变可能是引起胆囊癌发生发展的分子机制之一。%ObjectiveTo detect the expression of cyclinD1 and CDC25A in carcinoma of gallbladder tissue,to explore their mechanism, and to search for new therapy of carcinoma of gallbladder.Methods The expression degrees of cyclinD1 and CDC25A were examine in 44 cases of example carcinoma of gallbladder tissue and 35 cases of mucosa of chronic cholecystitis by FCM, the expression degrees of the protein were studied.Results The protein expression of in cyclinD1 carcinoma of gallbladder tissue and mucosa of chronic cholecystitis was 531.153±28.823 and 496.462±37.191 repectively,the protein expression of cyclinD1 was higher in carcinoma of gallbladder tissue than mucosa of chronic cholecystitis (P<0.05); The protein expression of CDC25A in carcinoma of gallbladder tissue and mucosa of chronic cholecystitis was 535.029±35.647 and 496.267±41.345 repectively,the protein expression of CDC25A was also higher in carcinoma of gallbladder tissue than mucosa of chronic cholecystitis (P<0.05). Conclusion The expression degree of protern was accordant with that of protein on CyclinD1 and CDC25A. It was higher in carcinoma of gallbladder tissue than in mucosa of chronic cholecystitis.Through the changes of cyclinD1 and CDC25A protein expression,it is possible to elucidate the carcinogenesis of cholangioncarcinoma.