[Objective] Cryopreservation is the best method in saving plant germplasm resources. [ Method ] Kuqa Apricot was used as test materials, and the preliminary choice of the tissue culture and hormone concentrations was made. [Result]The results showed that the hormone ratio of MS +0.5 NAA + 0. 2 6 - BA could make the callus obtained from stem aplex easily, and the root were most easily differentiated from stem tips induced callus on the level of MS + 0. 1 NAA +0. 1 6 -BA +0. 1GA. [ Conclusion] The comparative tests, combining vitrification method with different cryopreservation time, dehydration processing time and the thaw test method, indicated that pre - freezing temperature reaching - 30℃, liquid glass dehydrating 60 minutes and 40℃ water bath thawing within 5 minutes could make the survivability of cryopreservation reach the highest level.%[目的]采用玻璃化法对杏资源茎尖进行超低温保存.超低温保存是目前植物种质资源长期稳定保存的最好方法,保存后的材料遗传上较为稳定.[方法]以库车小白杏为试材,对茎尖(外植体)组织培养的培养基及激素浓度做了初步选择.[结果]选择MS +0.5NAA +0.2 6 -BA激素配方可使杏的茎尖最易形成愈伤材料,而选择MS +0.1 NAA +0.1 6- BA +0.1GA激素配方可使由茎尖获得的愈伤材料最易分化出根.[结论]用玻璃化法对组织培养获得的愈伤材料结合不同的低温保存时间、脱水处理时间与化冻方法进行比较试验可知,预冻温度- 30℃、玻璃化液保护剂脱水60 min、40℃水浴5 min解冻,可使超低温保存的材料复活率最高.
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