[目的]通过对陆地棉(Gossypium hirsutum L.)PHD(Plant homeodomain)转录因子在棉花不同组织和非生物胁迫条件下的表达分析,筛选棉花逆境胁迫应答基因.[方法]根据已报道的28个棉花GhPHD基因的EST序列合成了特异引物,使用半定量RT-PCR方法检测GhPHD在棉花种子、根、茎、叶、纤维(15DPA)中的表达以及在于旱、高盐(150 mmol/L NaCl)、低温(4℃)非生物胁迫下的表达.[结果]棉花PHD转录因子在各组织中的表达存在差异,85.7％的基因在种子中表达,在纤维中表达的占35.7％.在干旱胁迫条件下GhPHD1、GhPHD6和GhPHD22呈上调表达,在高盐胁迫条件下GhPHD1、GhPHD16呈上调表达,低温胁迫激活GhPHD9表达,其它基因对三种非生物胁迫无明显应答.[结论]部分GhPHDs在陆地棉应答和适应非生物胁迫过程中可能发挥重要的作用.%[Objective]In order to identify novel genes involved in upland cotton (Gossypium hirsutum L. ) , plant stress adaptation, expression of GhPHDs under different kinds of abiotic stresses, such as drought, high salt and low temperature were analyzed. [ Method ] According to the reported 28 GhPHDs, specific primers were synthesized. Semi - quantitative RT - PCR method was used to analyze expression patterns of GhPHDs in cotton seed, root, stem, leaf and fiber(15DPA)and under abiotic stresses such as drought, high salt (150 mmol/L NaCl) and low temperature (4℃). [Result] The 28 GhPHDs showed different tissue -specific expression patterns in seed, root, stem, leaf and fiber(15DPA) of cotton. The proportion of genes expressed in seed was 85.7% and 35.7% in fiber. These genes were differentially regulated by various abiotic stresses. The expressions of GhPHD1, GhPHD6 and GhPHD22 were activated by drought stress, and GhPHD1, GhPHD16 were up - regulated by high salt stress. GhPHD9 was up - regulated by low temperature. Other genes didnt show obvious response to these three abiotic stresses. [ Conclusion] It implies that GhPHDs may play important roles during upland cotton plant adapted to abiotic stresses.
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