两种表达载体对西瓜食酸菌生物学特性的影响

摘要

[Objective] This project aims to analyze the effects of two types of expression vectors on the biological characteristics ofAcidovorax citrulli in the hope of providing a theoretical reference for the study of gene function.[Method]The wild type FC440 strains of watermelon acid bacteria were transferred into the ex-pression vectors pBBR1MCS-5 and pHC60 respectively. Then the extracellular cellulase activity, extracellu-lar protease activity, extracellular polysaccharide content, mobility, pathogenicity and hypersensitive reaction were obtained and the effects of growth rate and copper resistance on the biological characteristics of the trans-formed strain were analyzed.[Result]The results showed that vector pBBR1MCS-5 had no significant effect on the biological characteristics ofAcidovorax citrulli, but vector pHC60 significantly inhibited the growth rate and motility of the strain. [Conclusion]During the study of gene function, the expression vector itself may af-fect some biological characteristics ofAcidovorax citrulli. It is necessary to choose an appropriate expression vector while exploring the function of a gene.%[目的]分析两种常用基因功能表达载体对宿主西瓜食酸菌生物学特性的影响,为基因功能研究选择载体提供理论参考.[方法]以西瓜食酸菌野生型FC440菌株为宿主菌,分别转入表达载体pBBR1MCS-5和pHC60,从胞外纤维素酶活性、胞外蛋白酶活性、胞外多糖含量、游动性、致病性和过敏性反应、生长速率以及抗铜性等,对转化菌株进行生物学特性影响的分析.[结果]载体pBBR1MCS-5对菌株的生物学特性各方面未表现出显著影响,载体pHC60会显著抑制菌株的生长速率和游动性.[结论]表达载体本身会影响西瓜食酸菌的某些生物学特性,在基因功能研究中,应依据所研究基因的功能选择适宜的表达载体.