基因芯片技术筛选吉西他滨诱导胰腺癌Panc-1细胞凋亡相关基因

摘要

目的 研究抗癌药吉西他滨(Gemcitabine)诱导胰腺癌Panc-1细胞凋亡的相关基因.方法 应用基因芯片技术检测50.33μg/L Gemcitabine作用于胰腺癌PANC-1细胞48h后凋亡基因表达的变化.结果 Gemcitabine抑制细胞表达14种凋亡相关基因,包括IRF-4、Scotin、14-3-3gamma、PDE4、GRPS、TID1、AFP、STAT5、Sox-4、c-Rel、MYCN、Htt、CatD、Gd.相反,gemcitabine促进了3种凋亡相关基因的表达,包括CyclinB1、ASK1、AKT2.结论 Gemcitabine诱导胰腺癌Panc-1细胞凋亡通过非依赖caspase ASK1途径.%Objective To study the molecular mechanisms of antitumor agent Gemcitabine on human carcinoma cells. Methods Gene expression profile was identified by cDNA microarrays on pancreatic carcinoma Panc-1 cells exposed to Gemcitabine(50.33mg/L) for 48hr. Results Gemcitabine-treated cells decreased expression of seventeen apoptotic genes. In contrast, these treated cells overexpressed three apoptotic genes. Conclusion It displayed that the apoptotic pathway could be caspase-independent ASK1 pathway.