目的:通过建立大鼠糖尿病性勃起功能障碍(Diabetic.Erectile.Dysfunction,DED)模型,研究右归胶囊对DED大鼠阴茎组织抗氧化能力及细胞凋亡的影响.方法:利用成年雄性SD大鼠建立糖尿病勃起功能障碍模型,将成模大鼠随机分为右归胶囊高剂量组、中剂量组、低剂量组及糖尿病组.给药12周后所有大鼠颈部皮下阿扑吗啡观察勃起情况并筛选ED模型,应用分光光度法及免疫组化方法检测阴茎海绵体组织中超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化氢酶(GSH-Px)及凋亡蛋白Bcl-2、Bax的表达.结果:各组之间勃起阳性率未见明显差异;右归胶囊高剂量组与糖尿病组相比,GSH含量增高、SOD、GSH-Px的活性提高;右归胶囊高剂量组与糖尿病组相比,Bcl-2阳性细胞率较高,Bax表达较低,Bcl-2/bax.比值较高.结论:右归胶囊可通过提高阴茎海绵体抗氧化能力并调控细胞凋亡,改善DM大鼠勃起功能障碍氧化损伤.%Objective:To investigate the antioxidant effect and impact on apoptosis of Yougui capsule on penis tissue in the diabetic rats. We established the diabetes mellitus (DM ) rat model of erectile dysfunftion(ED ) by injecting strcptozotoein(STZ ). Methods: A-dult male SD rats were randomly divided into four groups, high dose, medium dose and low dose of Yougui capsule and DM group. After 12 weeks, all rats were injected with apormorphinc (APO). We observed the results and selected the erectile dysfunction model; and also detected the content of SOD、GSH、GSH-PX and Bel-2、Bax with the application of speetrophotometry and immunohistoehemistry in rats corpus cavernous. Results: There was no difference between four groups in terms of the erection rate; In high dose group, the content of GSH,SOD,GSH-Px and the expression of Bcl-2, Bax and the ratio of Bcl-2/Bax were higher than those of the diabetes groups. Conclusion: Yougui capsule may help to treat erectile dysfunction in DM rats by enhancing antioxidant ability and regulating apoptosis of the penile corpus cavernous.
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