不同浓度艾烟长期干预对大鼠血清vWF及ox-LDL表达的影响

摘要

Objective:To observe the effect of moxa somke with different concentrations on blood microcirculation and provide reference on security of moxa smoke in clinic .Methods:A total of 168 rats were randomly divided into 4 groups ,42 in each group.Rats in con-trol group, low concentration goup, medium concentration group , high concentration group were respectively exposed to moxa smoke of 0%, 10%, 40%, 70%concentration (in terms of opacity) for 20 min each day , after 26 weeks rats were executed and drawn materi-als and with ELISA method ox -LDL、vWF in rats blood serum of different group were tested .Results: vWF showed downtrend as the concentration of moxa smoke ascended , which of low, medium, high concenteation group significantly declined compared with control group;It has significantly rise in medium cencentrtion group compared with control group ,the rest groups had no significant change by contrast with control group.Conclusion:1) The rise of concentration of moxa smoke brings down Platelet aggregation ,promotes body metabolism and improves microcirculation .2) Low concentration of moxa smoke indicates no injury on vascular endothelial .%目的：观察不同浓度艾烟对大鼠血液微循环的影响，为临床安全使用艾烟提供参考。方法：将168只wistar大鼠随机分为四组，每组42只分别暴露于0％、10％、40％、70％浓度（以染毒柜不透光率度量），每天20 min，26周后取材，ELISA法检测不同浓度组大鼠血清中vWF、ox-LDL的含量。结果：vWF含量随艾烟浓度升高而逐渐下降，低、中、高浓度组vWF含量与空白对照组相比均有统计学意义；中浓度组艾烟干预后，血清中ox-LDL含量显著性升高，其余各组ox-LDL含量与空白组相比无统计学意义。结论：1）艾烟可使血小板聚集程度下降，提示艾烟可能具有改善微循环，从而促进机体新陈代谢的作用。2）低浓度艾烟对血管内皮未见明显损伤。