基于代谢组学技术的白金胶囊治疗抑郁症的研究

摘要

Objective:To explore the mechanism of Baijin capsule in the treatment of depression based on metabolomics.Methods:SD rats modeled by Chronic Unpredictable Mild Stress were made as experimental objects.The effect of modeling and antidepressant efficacy of Baijin capsule was appraised before the study of brain metabolomics with the results of determination including the body weight,su-crose consumption and open field trial.Additionally,the study attempted to find out the biological information related to depression in samples of brain tissue based on brain metabolomics research.Pharmacokinetics research was obtained by the combined result of LC-MS/MS and analytical software SPSS/SIMACA-P and retrieval of database ( MASSBANK,METLIN,HMDB and KEGG) and literature analy-sis.Results:One thousand one hundred and ninty four compounds were determined by Metabolomics.The results of PCA and OPLS-DA indicated that the score matrix in normal group,CUMS group and drug group were distributed in three different areas which meant that there was a difference between groups.Four main indicators of compound taurine,threonic acid,N-acetylaspartate and inosine were identi-fied by integrating load matrix,library search and literature analysis.Conclusion:Baijin capsule is able to regulate the brain as an antide-pressant by adjusting the levels of amino acids,effecting on central nervous system of neurotransmitter and energy metabolism.%目的：利用代谢组学技术探讨白金胶囊治疗抑郁症的机制。方法：以SD大鼠为研究对象，采用慢性不可预知应激制备抑郁大鼠模型。在进行脑代谢组学研究之前，采用体质量、糖水消耗量、旷场试验测定结果为指标评价建模效果和白金胶囊抗抑郁疗效。脑代谢组学研究采用液质联用技术结合SPSS、SIMACA－P软件分析测定结果，通过数据库（ MASSBANK、METLIN、HMDB和KEGG）检索和文献分析，寻找出和抑郁相关的脑组织样本中的生物信息。结果：测得脑组织样本中共1194种化合物。 PCA和OPLS－DA分析结果显示，得分矩阵中的空白组、模型组和药物组分别集中分布在3个不同区域，说明存在组间差异；结合载荷矩阵和VIP值进行搜库查询及文献分析，得到牛磺酸、苏糖酸、N－乙酰天冬氨酸和肌苷4个主要化合物。结论：白金胶囊的抗抑郁药效与其改变脑内氨基酸水平、作用于中枢神经系统神经递质及参与能量代谢有关。